------------------------------------------------------------ Pratt version 2.1, Sept. 1996 Written by Inge Jonassen, University of Bergen Norway email: inge@ii.uib.no For more information, see http://www.ii.uib.no/~inge/Pratt.html ------------------------------------------------------------ Please quote: I.Jonassen, J.F.Collins, D.G.Higgins. Protein Science 1995;4(8):1587-1595. ------------------------------------------------------------ Pratt version 2.1 Analysing 7 sequences from file RIBOSOMAL_L35 PATTERN CONSERVATION: CM: min Nr of Seqs to Match 7 C%: min Percentage Seqs to Match 100.0 PATTERN RESTRICTIONS : PP: pos in seq [off,complete,start] off PL: max Pattern Length 50 PN: max Nr of Pattern Symbols 50 PX: max Nr of consecutive x's 5 FN: max Nr of flexible spacers 2 FL: max Flexibility 2 FP: max Flex.Product 10 BI: Input Pattern Symbol File off BN: Nr of Pattern Symbols Initial Search 20 PATTERN SCORING: S: Scoring [info,mdl,tree,dist,ppv] info SEARCH PARAMETERS: G: Pattern Graph from [seq,al,query] seq E: Search Greediness 3 R: Pattern Refinement on RG: Generalise ambiguous symbols off OUTPUT: OF: Output Filename RIBOSOMAL_L35.pratt2 OP: PROSITE Pattern Format on ON: max number patterns 20 OA: max number Alignments 20 M: Print Patterns in sequences off Sequence lengths: RL35_BACST 65 RL35_CYAPA 65 RL35_ECOLI 64 RL35_HAEIN 64 RL35_MYCFE 61 RL35_SPIOL 159 RL35_THETH 65 Pratt run started at Thu Feb 6 21:06:00 1997 Best Patterns before refinement: fitness hits(seqs) Pattern 1: 19.8503 9( 7) K-x(1,2)-K-x(0,1)-T-x(5)-K-R 2: 19.3503 7( 7) K-x-K-T-x(2,4)-A-x(0,1)-K 3: 15.6802 11( 7) K-T-x(3,5)-K-R 4: 15.6802 7( 7) K-x(2)-K-x(1,3)-K-R 5: 15.6802 7( 7) K-x(5)-G-x(2)-K-x(0,2)-R 6: 15.1802 9( 7) T-x(0,1)-K-x(1,3)-K-R 7: 15.1802 7( 7) H-L-x(2,3)-K-x(2,4)-K 8: 15.1802 7( 7) K-T-x(2,4)-A-x(0,1)-K 9: 14.6802 10( 7) K-x(0,2)-A-x(2,4)-K-R 10: 14.6802 8( 7) T-x(2,4)-A-x-K-x(0,2)-K 11: 12.0102 11( 7) K-R-x(0,1)-K 12: 12.0102 8( 7) K-x-T-x(2,3)-K 13: 12.0102 10( 7) A-x(1,2)-K-x(2)-K 14: 11.5102 8( 7) R-x(0,1)-K-x(1,2)-L 15: 11.5102 10( 7) H-x(4)-K-x(2,4)-K 16: 11.5102 8( 7) K-x(3,4)-H-x(3,4)-K 17: 11.5102 17( 7) K-x(3,4)-R-x(0,1)-K 18: 11.5102 8( 7) K-x-K-x(2,4)-A 19: 11.5102 9( 7) K-x(1,2)-K-x(1,2)-A 20: 11.5102 11( 7) K-x(2)-R-x(3,5)-K Best Patterns (after refinement phase): fitness hits(seqs) Pattern A 1: 60.7255 7( 7) K-x-K-T-x(2)-[AG]-x(2)-K-R-x-[KR]-x-[TV]-[AG]-x(3)-[FILV]-x(4)-[AST]-x(2)-[QRS]-H-[IL]-x(3)-K-[ANST]-x(2)-[EQR]-x-[NR] B 2: 52.3854 7( 7) K-T-x(3,5)-K-R-x-[KR]-x-[TV]-[AG]-x(3)-[FILV]-x(4)-[AST]-x(2)-[QRS]-H-[IL]-x(3)-K-[ANST]-x(2)-[EQR]-x-[NR] C 3: 29.8091 7( 7) K-R-x(0,1)-K-x(2)-[AGL]-x(3)-[FILV]-x(4)-[AST]-x(2)-[DRS]-x-[ILV]-x(4)-[ANPST]-x(4)-[NR] D 4: 28.0962 7( 7) K-[ILM]-K-T-x(2,4)-A-x(0,1)-K-[KR]-x(4)-[AGT] E 5: 21.0373 7( 7) K-T-x(2,4)-A-x(0,1)-K-[KR]-x(4)-[AGT] F 6: 18.3343 7( 7) K-[ANT]-x-K-x(1,3)-K-R G 7: 16.9741 7( 7) H-[IL]-x(3)-K-x(2,4)-K-[ENQR] H 8: 15.6802 7( 7) K-x(5)-G-x(2)-K-x(0,2)-R I 9: 15.1802 9( 7) T-x(0,1)-K-x(1,3)-K-R J 10: 15.1802 7( 7) H-L-x(2,3)-K-x(2,4)-K K 11: 14.6802 10( 7) K-x(0,2)-A-x(2,4)-K-R L 12: 14.6802 8( 7) T-x(2,4)-A-x-K-x(0,2)-K M 13: 14.3991 7( 7) K-[ILM]-K-x(2,4)-A N 14: 12.0102 8( 7) K-x-T-x(2,3)-K O 15: 12.0102 10( 7) A-x(1,2)-K-x(2)-K P 16: 11.5102 11( 7) K-T-x(3,5)-K Q 17: 11.5102 8( 7) R-x(0,1)-K-x(1,2)-L R 18: 11.5102 8( 7) K-x(3,4)-H-x(3,4)-K S 19: 11.5102 17( 7) K-x(3,4)-R-x(0,1)-K T 20: 11.5102 8( 7) K-x-K-x(2,4)-A Best patterns with alignements: fitness hits(seqs) Pattern A 1: 60.7255 7( 7) K-x-K-T-x(2)-[AG]-x(2)-K-R-x-[KR]-x-[TV]-[AG]-x(3)-[FILV]-x(4)-[AST]-x(2)-[QRS]-H-[IL]-x(3)-K-[ANST]-x(2)-[EQR]-x-[NR] Occurences: 7(7) RL35_BACST : 2- 41: p KmKThrGsaKRfKkTAsgkLkrghAytSHLfanKTkkQkR hlrka RL35_CYAPA : 3- 42: my KlKTrkAaaKRyKaVGnkkIsrrkAfrSHLlqkKStnRkR qlsqv RL35_ECOLI : 2- 41: p KiKTvrGasKRfKkTGkggFkhkhAnlRHIltkKAtkRkR hlrpk RL35_HAEIN : 2- 41: p KiKTvrGaaKRfKkTAsggFkrkqShlRHIfdxKNtkRkR hlrhk RL35_MYCFE : 2- 41: p KmKTksAlkKRiKiTGtgkImreqAyrSHLsqnKTtkQkR qarks RL35_SPIOL : 91- 130: aakgy KmKThkAsaKRfRvTGkgkIvrrrAgkQHLlakKNtkRkN rlskl RL35_THETH : 2- 41: p KmKThkGakKRvKiTAsgkVvamkTgkRHLnwqKSgkEiR qkgrk B 2: 52.3854 7( 7) K-T-x(3,5)-K-R-x-[KR]-x-[TV]-[AG]-x(3)-[FILV]-x(4)-[AST]-x(2)-[QRS]-H-[IL]-x(3)-K-[ANST]-x(2)-[EQR]-x-[NR] Occurences: 7(7) RL35_BACST : 4- 41: pkm KThrgsaKRfKkTAsgkLkrghAytSHLfanKTkkQkR hlrka RL35_CYAPA : 5- 42: mykl KTrkaaaKRyKaVGnkkIsrrkAfrSHLlqkKStnRkR qlsqv RL35_ECOLI : 4- 41: pki KTvrgasKRfKkTGkggFkhkhAnlRHIltkKAtkRkR hlrpk RL35_HAEIN : 4- 41: pki KTvrgaaKRfKkTAsggFkrkqShlRHIfdxKNtkRkR hlrhk RL35_MYCFE : 4- 41: pkm KTksalkKRiKiTGtgkImreqAyrSHLsqnKTtkQkR qarks RL35_SPIOL : 93- 130: kgykm KThkasaKRfRvTGkgkIvrrrAgkQHLlakKNtkRkN rlskl RL35_THETH : 4- 41: pkm KThkgakKRvKiTAsgkVvamkTgkRHLnwqKSgkEiR qkgrk C 3: 29.8091 7( 7) K-R-x(0,1)-K-x(2)-[AGL]-x(3)-[FILV]-x(4)-[AST]-x(2)-[DRS]-x-[ILV]-x(4)-[ANPST]-x(4)-[NR] Occurences: 7(7) RL35_BACST : 11- 41: hrgsa KRfKktAsgkLkrghAytShLfankTkkqkR hlrka RL35_CYAPA : 12- 42: rkaaa KRyKavGnkkIsrrkAfrShLlqkkStnrkR qlsqv RL35_ECOLI : 11- 41: vrgas KRfKktGkggFkhkhAnlRhIltkkAtkrkR hlrpk RL35_HAEIN : 11- 41: vrgaa KRfKktAsggFkrkqShlRhIfdxkNtkrkR hlrhk RL35_MYCFE : 11- 41: ksalk KRiKitGtgkImreqAyrShLsqnkTtkqkR qarks RL35_SPIOL : 127- 156: akknt KR-KnrLsklIqvdrSdyDnVigalPylkvN rkv RL35_THETH : 11- 41: hkgak KRvKitAsgkVvamkTgkRhLnwqkSgkeiR qkgrk D 4: 28.0962 7( 7) K-[ILM]-K-T-x(2,4)-A-x(0,1)-K-[KR]-x(4)-[AGT] Occurences: 7(7) RL35_BACST : 2- 17: p KMKThrgsA-KRfkktA sgklk RL35_CYAPA : 3- 18: my KLKTrkaaA-KRykavG nkkis RL35_CYAPA : 3- 18: my KLKTrka-AaKRykavG nkkis RL35_ECOLI : 2- 17: p KIKTvrg-AsKRfkktG kggfk RL35_HAEIN : 2- 17: p KIKTvrgaA-KRfkktA sggfk RL35_HAEIN : 2- 17: p KIKTvrg-AaKRfkktA sggfk RL35_MYCFE : 2- 16: p KMKTks--AlKKrikiT gtgki RL35_SPIOL : 91- 106: aakgy KMKThkasA-KRfrvtG kgkiv RL35_THETH : 2- 16: p KMKThkg-A-KKrvkiT asgkv RL35_THETH : 2- 17: p KMKThkg-AkKRvkitA sgkvv E 5: 21.0373 7( 7) K-T-x(2,4)-A-x(0,1)-K-[KR]-x(4)-[AGT] Occurences: 7(7) RL35_BACST : 4- 17: pkm KThrgsA-KRfkktA sgklk RL35_CYAPA : 5- 18: mykl KTrkaaA-KRykavG nkkis RL35_CYAPA : 5- 18: mykl KTrka-AaKRykavG nkkis RL35_ECOLI : 4- 17: pki KTvrg-AsKRfkktG kggfk RL35_HAEIN : 4- 17: pki KTvrgaA-KRfkktA sggfk RL35_HAEIN : 4- 17: pki KTvrg-AaKRfkktA sggfk RL35_MYCFE : 4- 16: pkm KTks--AlKKrikiT gtgki RL35_SPIOL : 93- 106: kgykm KThkasA-KRfrvtG kgkiv RL35_THETH : 4- 16: pkm KThkg-A-KKrvkiT asgkv RL35_THETH : 4- 17: pkm KThkg-AkKRvkitA sgkvv F 6: 18.3343 7( 7) K-[ANT]-x-K-x(1,3)-K-R Occurences: 7(7) RL35_BACST : 35- 41: hlfan KTkKq--KR hlrka RL35_CYAPA : 5- 13: mykl KTrKaaaKR ykavg RL35_ECOLI : 35- 41: hiltk KAtKr--KR hlrpk RL35_HAEIN : 35- 41: hifdx KNtKr--KR hlrhk RL35_MYCFE : 35- 41: hlsqn KTtKq--KR qarks RL35_SPIOL : 93- 101: kgykm KThKasaKR frvtg RL35_THETH : 4- 12: pkm KThKgakKR vkita G 7: 16.9741 7( 7) H-[IL]-x(3)-K-x(2,4)-K-[ENQR] Occurences: 7(7) RL35_BACST : 30- 39: hayts HLfanKtk--KQ krhlr RL35_BACST : 30- 41: hayts HLfanKtkkqKR hlrka RL35_CYAPA : 31- 42: kafrs HLlqkKstnrKR qlsqv RL35_ECOLI : 30- 39: hanlr HIltkKat--KR krhlr RL35_ECOLI : 30- 41: hanlr HIltkKatkrKR hlrpk RL35_HAEIN : 30- 39: qshlr HIfdxKnt--KR krhlr RL35_HAEIN : 30- 41: qshlr HIfdxKntkrKR hlrhk RL35_MYCFE : 30- 39: qayrs HLsqnKtt--KQ krqar RL35_MYCFE : 30- 41: qayrs HLsqnKttkqKR qarks RL35_SPIOL : 119- 128: ragkq HLlakKnt--KR knrls RL35_SPIOL : 119- 130: ragkq HLlakKntkrKN rlskl RL35_THETH : 30- 39: ktgkr HLnwqKsg--KE irqkg H 8: 15.6802 7( 7) K-x(5)-G-x(2)-K-x(0,2)-R Occurences: 7(7) RL35_BACST : 2- 12: p KmkthrGsaK--R fkkta RL35_CYAPA : 12- 24: rkaaa KrykavGnkKisR rkafr RL35_ECOLI : 2- 12: p KiktvrGasK--R fkktg RL35_HAEIN : 2- 12: p KiktvrGaaK--R fkkta RL35_MYCFE : 11- 23: ksalk KrikitGtgKimR eqayr RL35_SPIOL : 100- 112: hkasa KrfrvtGkgKivR rragk RL35_THETH : 2- 12: p KmkthkGakK--R vkita I 9: 15.1802 9( 7) T-x(0,1)-K-x(1,3)-K-R Occurences: 9(7) RL35_BACST : 36- 41: lfank TkKq--KR hlrka RL35_BACST : 36- 41: lfank T-Kkq-KR hlrka RL35_CYAPA : 6- 13: myklk TrKaaaKR ykavg RL35_ECOLI : 33- 39: lrhil TkKat-KR krhlr RL35_ECOLI : 33- 39: lrhil T-KkatKR krhlr RL35_ECOLI : 37- 41: ltkka T-Kr--KR hlrpk RL35_HAEIN : 37- 41: fdxkn T-Kr--KR hlrhk RL35_MYCFE : 36- 41: lsqnk TtKq--KR qarks RL35_MYCFE : 37- 41: sqnkt T-Kq--KR qarks RL35_SPIOL : 94- 101: gykmk ThKasaKR frvtg RL35_THETH : 5- 12: pkmk ThKgakKR vkita J 10: 15.1802 7( 7) H-L-x(2,3)-K-x(2,4)-K Occurences: 7(7) RL35_BACST : 30- 38: hayts HLfanKtk--K qkrhl RL35_BACST : 30- 40: hayts HLfanKtkkqK rhlrk RL35_CYAPA : 31- 41: kafrs HLlqkKstnrK rqlsq RL35_ECOLI : 42- 51: tkrkr HLrp-KamvsK gdlgl RL35_HAEIN : 42- 51: tkrkr HLrh-KsmvaK adqvl RL35_MYCFE : 30- 38: qayrs HLsqnKtt--K qkrqa RL35_MYCFE : 30- 40: qayrs HLsqnKttkqK rqark RL35_SPIOL : 119- 127: ragkq HLlakKnt--K rknrl RL35_SPIOL : 119- 127: ragkq HLla-Kknt-K rknrl RL35_SPIOL : 119- 129: ragkq HLlakKntkrK nrlsk RL35_THETH : 30- 38: ktgkr HLnwqKsg--K eirqk K 11: 14.6802 10( 7) K-x(0,2)-A-x(2,4)-K-R Occurences: 10(7) RL35_BACST : 14- 23: sakrf KktAsgklKR ghayt RL35_BACST : 15- 23: akrfk Kt-AsgklKR ghayt RL35_CYAPA : 8- 13: klktr K--Aaa--KR ykavg RL35_ECOLI : 34- 41: rhilt Kk-Atkr-KR hlrpk RL35_ECOLI : 35- 41: hiltk K--Atkr-KR hlrpk RL35_HAEIN : 14- 23: aakrf KktAsggfKR kqshl RL35_HAEIN : 15- 23: akrfk Kt-AsggfKR kqshl RL35_MYCFE : 6- 12: pkmkt Ks-Alk--KR ikitg RL35_SPIOL : 96- 101: kmkth K--Asa--KR frvtg RL35_THETH : 20- 29: itasg KvvAmktgKR hlnwq L 12: 14.6802 8( 7) T-x(2,4)-A-x-K-x(0,2)-K Occurences: 8(7) RL35_BACST : 28- 37: rghay TshlfAnKt-K kqkrh RL35_BACST : 28- 38: rghay TshlfAnKtkK qkrhl RL35_CYAPA : 6- 15: myklk Trka-AaKryK avgnk RL35_ECOLI : 5- 14: pkik Tvrg-AsKrfK ktgkg RL35_ECOLI : 33- 40: lrhil Tkk--AtKr-K rhlrp RL35_HAEIN : 5- 14: pkik Tvrg-AaKrfK ktasg RL35_MYCFE : 5- 11: pkmk Tks--AlK--K rikit RL35_SPIOL : 83- 91: sspsf Tvf--AaKgyK mkthk RL35_THETH : 5- 14: pkmk Thkg-AkKrvK itasg M 13: 14.3991 7( 7) K-[ILM]-K-x(2,4)-A Occurences: 7(7) RL35_BACST : 20- 26: ktasg KLKrgh-A ytshl RL35_CYAPA : 3- 9: my KLKtrk-A aakry RL35_CYAPA : 3- 10: my KLKtrkaA akryk RL35_ECOLI : 2- 9: p KIKtvrgA skrfk RL35_HAEIN : 2- 9: p KIKtvrgA akrfk RL35_MYCFE : 2- 8: p KMKtks-A lkkri RL35_SPIOL : 91- 97: aakgy KMKthk-A sakrf RL35_THETH : 2- 9: p KMKthkgA kkrvk N 14: 12.0102 8( 7) K-x-T-x(2,3)-K Occurences: 8(7) RL35_BACST : 14- 20: sakrf KkTasgK lkrgh RL35_CYAPA : 36- 41: hllqk KsTnr-K rqlsq RL35_ECOLI : 35- 40: hiltk KaTkr-K rhlrp RL35_HAEIN : 35- 40: hifdx KnTkr-K rhlrh RL35_MYCFE : 14- 20: lkkri KiTgtgK imreq RL35_MYCFE : 35- 40: hlsqn KtTkq-K rqark RL35_SPIOL : 124- 129: hllak KnTkr-K nrlsk RL35_THETH : 14- 20: akkrv KiTasgK vvamk O 15: 12.0102 10( 7) A-x(1,2)-K-x(2)-K Occurences: 10(7) RL35_BACST : 33- 38: tshlf An-KtkK qkrhl RL35_CYAPA : 9- 15: lktrk AaaKryK avgnk RL35_CYAPA : 10- 15: ktrka Aa-KryK avgnk RL35_ECOLI : 9- 14: ktvrg As-KrfK ktgkg RL35_HAEIN : 9- 14: ktvrg Aa-KrfK ktasg RL35_MYCFE : 8- 14: mktks AlkKriK itgtg RL35_SPIOL : 86- 91: sftvf Aa-KgyK mkthk RL35_SPIOL : 122- 127: kqhll Ak-KntK rknrl RL35_THETH : 9- 14: kthkg Ak-KrvK itasg RL35_THETH : 23- 28: sgkvv Am-KtgK rhlnw P 16: 11.5102 11( 7) K-T-x(3,5)-K Occurences: 11(7) RL35_BACST : 4- 11: pkm KThrgsaK rfkkt RL35_BACST : 15- 22: akrfk KTasgklK rghay RL35_BACST : 35- 40: hlfan KTkkq--K rhlrk RL35_CYAPA : 5- 12: mykl KTrkaaaK rykav RL35_ECOLI : 4- 11: pki KTvrgasK rfkkt RL35_HAEIN : 4- 11: pki KTvrgaaK rfkkt RL35_HAEIN : 15- 22: akrfk KTasggfK rkqsh RL35_MYCFE : 4- 11: pkm KTksalkK rikit RL35_MYCFE : 35- 40: hlsqn KTtkq--K rqark RL35_SPIOL : 93- 100: kgykm KThkasaK rfrvt RL35_THETH : 4- 11: pkm KThkgakK rvkit Q 17: 11.5102 8( 7) R-x(0,1)-K-x(1,2)-L Occurences: 8(7) RL35_BACST : 44- 48: qkrhl R-KatL vspgd RL35_CYAPA : 40- 44: kkstn R-KrqL sqvvi RL35_ECOLI : 39- 43: kkatk R-KrhL rpkam RL35_HAEIN : 39- 43: xkntk R-KrhL rhksm RL35_MYCFE : 56- 60: ssdvk RfKa-L i RL35_SPIOL : 128- 132: kkntk R-KnrL skliq RL35_THETH : 45- 49: irqkg R-KfvL akpea RL35_THETH : 56- 60: kpeae RiKl-L lpyxr RL35_THETH : 56- 61: kpeae RiKllL pyxr R 18: 11.5102 8( 7) K-x(3,4)-H-x(3,4)-K Occurences: 8(7) RL35_BACST : 2- 11: p Kmkt-HrgsaK rfkkt RL35_CYAPA : 26- 35: kisrr KafrsHllq-K kstnr RL35_CYAPA : 26- 36: kisrr KafrsHllqkK stnrk RL35_ECOLI : 38- 46: tkkat Krkr-Hlrp-K amvsk RL35_HAEIN : 38- 46: dxknt Krkr-Hlrh-K smvak RL35_MYCFE : 45- 55: krqar KsvqmHssdvK rfkal RL35_SPIOL : 91- 100: aakgy Kmkt-HkasaK rfrvt RL35_THETH : 2- 10: p Kmkt-Hkga-K krvki RL35_THETH : 2- 11: p Kmkt-HkgakK rvkit RL35_THETH : 25- 35: kvvam KtgkrHlnwqK sgkei S 19: 11.5102 17( 7) K-x(3,4)-R-x(0,1)-K Occurences: 17(7) RL35_BACST : 40- 45: ktkkq Krhl-R-K atlvs RL35_CYAPA : 3- 8: my Klkt-R-K aaakr RL35_CYAPA : 8- 15: klktr KaaakRyK avgnk RL35_CYAPA : 20- 26: kavgn KkisrR-K afrsh RL35_CYAPA : 20- 26: kavgn Kkis-RrK afrsh RL35_CYAPA : 21- 26: avgnk Kisr-R-K afrsh RL35_CYAPA : 35- 41: shllq KkstnR-K rqlsq RL35_CYAPA : 36- 41: hllqk Kstn-R-K rqlsq RL35_ECOLI : 34- 40: rhilt KkatkR-K rhlrp RL35_ECOLI : 35- 40: hiltk Katk-R-K rhlrp RL35_ECOLI : 40- 46: katkr Krhl-RpK amvsk RL35_HAEIN : 35- 40: hifdx Kntk-R-K rhlrh RL35_HAEIN : 40- 46: kntkr Krhl-RhK smvak RL35_MYCFE : 40- 45: kttkq Krqa-R-K svqmh RL35_SPIOL : 123- 129: qhlla KkntkR-K nrlsk RL35_SPIOL : 124- 129: hllak Kntk-R-K nrlsk RL35_THETH : 7- 14: kmkth KgakkRvK itasg RL35_THETH : 51- 58: kfvla KpeaeRiK lllpy T 20: 11.5102 8( 7) K-x-K-x(2,4)-A Occurences: 8(7) RL35_BACST : 20- 26: ktasg KlKrgh-A ytshl RL35_CYAPA : 3- 9: my KlKtrk-A aakry RL35_CYAPA : 3- 10: my KlKtrkaA akryk RL35_ECOLI : 2- 9: p KiKtvrgA skrfk RL35_HAEIN : 2- 9: p KiKtvrgA akrfk RL35_MYCFE : 2- 8: p KmKtks-A lkkri RL35_MYCFE : 38- 43: qnktt KqKrq--A rksvq RL35_SPIOL : 91- 97: aakgy KmKthk-A sakrf RL35_THETH : 2- 9: p KmKthkgA kkrvk Number of patterns evaluated by Pratt:277 Total running time: 0 seconds