------------------------------------------------------------ Pratt version 2.1, Sept. 1996 Written by Inge Jonassen, University of Bergen Norway email: inge@ii.uib.no For more information, see http://www.ii.uib.no/~inge/Pratt.html ------------------------------------------------------------ Please quote: I.Jonassen, J.F.Collins, D.G.Higgins. Protein Science 1995;4(8):1587-1595. ------------------------------------------------------------ Pratt version 2.1 Analysing 8 sequences from file ENDONUCLEASE_III_1 PATTERN CONSERVATION: CM: min Nr of Seqs to Match 8 C%: min Percentage Seqs to Match 100.0 PATTERN RESTRICTIONS : PP: pos in seq [off,complete,start] off PL: max Pattern Length 50 PN: max Nr of Pattern Symbols 50 PX: max Nr of consecutive x's 5 FN: max Nr of flexible spacers 2 FL: max Flexibility 2 FP: max Flex.Product 10 BI: Input Pattern Symbol File off BN: Nr of Pattern Symbols Initial Search 20 PATTERN SCORING: S: Scoring [info,mdl,tree,dist,ppv] info SEARCH PARAMETERS: G: Pattern Graph from [seq,al,query] seq E: Search Greediness 3 R: Pattern Refinement on RG: Generalise ambiguous symbols off OUTPUT: OF: Output Filename ENDONUCLEASE_III_1.pratt2 OP: PROSITE Pattern Format on ON: max number patterns 20 OA: max number Alignments 20 M: Print Patterns in sequences off Sequence lengths: END3_BACSU 219 END3_ECOLI 211 END3_HAEIN 211 GTMR_METTF 221 MUTY_ECOLI 350 MUTY_HAEIN 378 MUTY_SALTY 350 UVEN_MICLU 268 Pratt run started at Thu Feb 6 19:25:57 1997 Best Patterns before refinement: fitness hits(seqs) Pattern 1: 24.5203 8( 8) L-x-G-V-G-x(2)-T-x(0,1)-A 2: 20.8503 8( 8) C-x(4)-P-x-C-x(2)-C-x(5)-C 3: 20.3503 8( 8) G-V-G-x(2)-T-x(0,1)-A 4: 16.6802 8( 8) P-x-C-x(2)-C-x(5)-C 5: 16.1802 8( 8) A-x(2)-V-x(5)-G-x(1,2)-P 6: 16.1802 8( 8) V-G-x(2)-T-x(0,1)-A 7: 15.6802 10( 8) L-x(3,4)-G-x(2)-T-x(0,1)-A 8: 15.6802 8( 8) K-x(4)-V-x(5)-G-x(1,3)-P 9: 15.6802 8( 8) K-x(4,5)-V-x(5)-G-x(1,2)-P 10: 15.6802 8( 8) L-x(1,2)-L-x(1,2)-G-x-G 11: 15.6802 8( 8) L-x(3,4)-G-x-G-x(3,4)-A 12: 15.1802 10( 8) L-x(2)-V-x(2,3)-T-x(0,2)-A 13: 14.6802 10( 8) L-x-G-x(2,4)-T-x(0,2)-A 14: 14.6802 12( 8) L-x(2,4)-R-x-T-x(0,2)-A 15: 14.6802 8( 8) G-x-V-x(1,3)-R-x(1,3)-L 16: 12.5102 8( 8) C-x(2)-C-x(5)-C 17: 12.5102 8( 8) D-x(4)-R-x(3)-R 18: 12.5102 8( 8) R-x-T-A 19: 12.5102 9( 8) G-V-x(3)-T 20: 12.0102 10( 8) G-x-Y-x(2,3)-A Best Patterns (after refinement phase): fitness hits(seqs) Pattern A 1: 74.8284 8( 8) G-V-G-[KR]-x-T-[AC]-x-[AV]-[IV]-[LMV]-[CGNS]-x-[ASV]-[FL]-[GN]-x(3)-[AIP]-x-[LV]-D-[AGT]-x-[FIV]-x-R-[LV]-x(2)-R-x(3)-[ACGTV]-x(3)-[DNPS] B 2: 36.3695 8( 8) L-[APS]-G-V-G-[KR]-x-T-x(0,1)-A-x-[AV]-[IMV] C 3: 35.5650 8( 8) L-x(1,2)-L-x(1,2)-G-[LV]-G-x(2)-[AT]-x(3)-[NV]-[LMV]-x(2)-A-x-[GQ] D 4: 29.5804 8( 8) G-V-G-[KR]-x-T-x(0,1)-A-x-[AV]-[IMV] E 5: 28.0509 8( 8) C-x(4)-P-x-C-[ADEGST]-x-C-[GIP]-[ILM]-x(3)-C F 6: 27.1316 8( 8) L-x(3,4)-G-[LV]-G-x(3,4)-A-x-[NV]-[LMV]-x(2)-[ANTV] G 7: 26.2285 8( 8) G-[ENR]-V-x(1,3)-R-x(1,3)-L-x(4)-[AGV]-[GV]-x(4)-[AG] H 8: 25.4104 8( 8) V-G-[KR]-x-T-x(0,1)-A-x-[AV]-[IMV] I 9: 25.4104 8( 8) L-x(3)-G-[KR]-x-T-x(0,1)-A-x-[AV]-[IMV] J 10: 25.1530 8( 8) L-[APS]-G-V-x(2,3)-T-x(0,2)-A-x-[LV] K 11: 25.0577 8( 8) D-[AGT]-x-[FIV]-x-R-[LV]-x(2)-R-x(3)-[ACGTV]-x(3)-[DNPS] L 12: 23.8809 8( 8) P-x-C-[ADEGST]-x-C-[GIP]-[ILM]-x(3)-C M 13: 23.7241 8( 8) K-[AET]-[AL]-x(2)-V-x(5)-G-x(1,3)-P-x(4)-[ADEQ] N 14: 20.4830 8( 8) L-[APS]-G-x(2,4)-T-x(0,2)-A-x-[LV] O 15: 20.3307 8( 8) A-x(2)-V-x-[DGNST]-x(3)-G-x(1,2)-P-x(4)-[ADEQ] P 16: 19.8307 8( 8) K-x(4,5)-V-x-[DGNST]-x(3)-G-x(1,2)-P-x(4)-[ADEQ] Q 17: 19.7108 8( 8) C-[ADEGST]-x-C-[GIP]-[ILM]-x(3)-C R 18: 18.9051 8( 8) L-x(2,4)-R-x-T-A-x(2)-[IV] S 19: 15.7350 8( 8) R-x-T-A-x(2)-[IV] T 20: 14.6802 8( 8) G-x-V-x(1,3)-R-x(1,3)-L Best patterns with alignements: fitness hits(seqs) Pattern A 1: 74.8284 8( 8) G-V-G-[KR]-x-T-[AC]-x-[AV]-[IV]-[LMV]-[CGNS]-x-[ASV]-[FL]-[GN]-x(3)-[AIP]-x-[LV]-D-[AGT]-x-[FIV]-x-R-[LV]-x(2)-R-x(3)-[ACGTV]-x(3)-[DNPS] Occurences: 8(8) END3_BACSU : 117- 156: lvklp GVGRkTAnVVVSvAFGvpaIaVDThVeRVskRlgiCrwkD svlev END3_ECOLI : 116- 155: lealp GVGRkTAnVVLNtAFGwptIaVDThIfRVcnRtqfApgkN veqve END3_HAEIN : 116- 155: leala GVGRkTAnVVLNtAFGhptIaVDThIfRVcnRtnfAagkD vvkve GTMR_METTF : 122- 161: ildlp GVGKyTCaAVMClAFGkkaAmVDAnFvRVinRyfgGsyeN lnynh MUTY_ECOLI : 116- 155: vaalp GVGRsTAgAILSlSLGkhfPiLDGnVkRVlaRcyaVsgwP gkkev MUTY_HAEIN : 121- 160: vwals GVGRsTAgAILSsVLNqpyPiLDGnVkRVlaRyfaVegwS gekkv MUTY_SALTY : 116- 155: iaalp GVGRsTAgAILSlALGkhyPiLDGnVkRVlaRcyaVsgwP gkkev UVEN_MICLU : 131- 170: lvalp GVGRkTAfVVLGnAFGqpgItVDThFgRLarRlgfTdetD pgkgr B 2: 36.3695 8( 8) L-[APS]-G-V-G-[KR]-x-T-x(0,1)-A-x-[AV]-[IMV] Occurences: 8(8) END3_BACSU : 115- 126: delvk LPGVGRkT-AnVV vsvaf END3_ECOLI : 114- 125: aalea LPGVGRkT-AnVV lntaf END3_HAEIN : 114- 125: ealea LAGVGRkT-AnVV lntaf GTMR_METTF : 120- 132: kaild LPGVGKyTcAaVM clafg MUTY_ECOLI : 114- 125: eevaa LPGVGRsT-AgAI lslsl MUTY_HAEIN : 119- 130: eqvwa LSGVGRsT-AgAI lssvl MUTY_SALTY : 114- 125: aeiaa LPGVGRsT-AgAI lslal UVEN_MICLU : 129- 140: edlva LPGVGRkT-AfVV lgnaf C 3: 35.5650 8( 8) L-x(1,2)-L-x(1,2)-G-[LV]-G-x(2)-[AT]-x(3)-[NV]-[LMV]-x(2)-A-x-[GQ] Occurences: 8(8) END3_BACSU : 112- 132: rdrde LvkLp-GVGrkTanvVVsvAfG vpaia END3_ECOLI : 111- 131: edraa LeaLp-GVGrkTanvVLntAfG wptia END3_HAEIN : 111- 131: enrea LeaLa-GVGrkTanvVLntAfG hptia GTMR_METTF : 118- 137: nrkai Ld-Lp-GVGkyTcaaVMclAfG kkaam MUTY_ECOLI : 74- 94: pldev Lh-LwtGLGyyArarNLhkAaQ qvatl MUTY_HAEIN : 79- 99: sqdev Lh-LwtGLGyyArarNLhkAaQ kvrde MUTY_SALTY : 74- 94: pldev Lh-LwtGLGyyArarNLhkAaQ qvatl UVEN_MICLU : 126- 146: arled LvaLp-GVGrkTafvVLgnAfG qpgit D 4: 29.5804 8( 8) G-V-G-[KR]-x-T-x(0,1)-A-x-[AV]-[IMV] Occurences: 8(8) END3_BACSU : 117- 126: lvklp GVGRkT-AnVV vsvaf END3_ECOLI : 116- 125: lealp GVGRkT-AnVV lntaf END3_HAEIN : 116- 125: leala GVGRkT-AnVV lntaf GTMR_METTF : 122- 132: ildlp GVGKyTcAaVM clafg MUTY_ECOLI : 116- 125: vaalp GVGRsT-AgAI lslsl MUTY_HAEIN : 121- 130: vwals GVGRsT-AgAI lssvl MUTY_SALTY : 116- 125: iaalp GVGRsT-AgAI lslal UVEN_MICLU : 131- 140: lvalp GVGRkT-AfVV lgnaf E 5: 28.0509 8( 8) C-x(4)-P-x-C-[ADEGST]-x-C-[GIP]-[ILM]-x(3)-C Occurences: 8(8) END3_BACSU : 189- 205: fgryh CkaqsPrCAeCPLlslC regqk END3_ECOLI : 187- 203: hgryt CiarkPrCGsCIIedlC eykek END3_HAEIN : 187- 203: hgryt CiarkPrCGsCIIedlC eykek GTMR_METTF : 197- 213: fsaii CaprkPkCEkCGMsklC syyek MUTY_ECOLI : 192- 208: lgami CtrskPkCSlCPLqngC iaaan MUTY_HAEIN : 197- 213: igamv CmrtkPkCDlCPLnidC laykn MUTY_SALTY : 192- 208: lgamv CtrskPkCTlCPLqngC iaaah UVEN_MICLU : 203- 219: hgrrv CharrPaCGrCPIarwC psyaa F 6: 27.1316 8( 8) L-x(3,4)-G-[LV]-G-x(3,4)-A-x-[NV]-[LMV]-x(2)-[ANTV] Occurences: 8(8) END3_BACSU : 112- 129: rdrde LvklpGVGrkt-AnVVvsV afgvp END3_ECOLI : 111- 128: edraa LealpGVGrkt-AnVVlnT afgwp END3_HAEIN : 111- 128: enrea LealaGVGrkt-AnVVlnT afghp GTMR_METTF : 118- 135: nrkai Ldlp-GVGkytcAaVMclA fgkka MUTY_ECOLI : 74- 92: pldev LhlwtGLGyyarArNLhkA aqqva MUTY_HAEIN : 79- 97: sqdev LhlwtGLGyyarArNLhkA aqkvr MUTY_SALTY : 74- 92: pldev LhlwtGLGyyarArNLhkA aqqva UVEN_MICLU : 126- 143: arled LvalpGVGrkt-AfVVlgN afgqp G 7: 26.2285 8( 8) G-[ENR]-V-x(1,3)-R-x(1,3)-L-x(4)-[AGV]-[GV]-x(4)-[AG] Occurences: 8(8) END3_BACSU : 103- 123: iedyg GEVprdRde-LvklpGVgrktA nvvvs END3_ECOLI : 102- 122: leqhn GEVpedRaa-LealpGVgrktA nvvln END3_HAEIN : 102- 122: iekhn GEVpenRea-LealaGVgrktA nvvln GTMR_METTF : 108- 129: indyg GRVprnRkaiLdlpgVGkytcA avmcl MUTY_ECOLI : 139- 156: fpild GNVk--Rv--LarcyAVsgwpG kkeve MUTY_HAEIN : 144- 161: ypild GNVk--Rv--LaryfAVegwsG ekkve MUTY_SALTY : 139- 156: ypild GNVk--Rv--LarcyAVsgwpG kkeve UVEN_MICLU : 117- 137: vgrhd GEVpa-RledLvalpGVgrktA fvvlg H 8: 25.4104 8( 8) V-G-[KR]-x-T-x(0,1)-A-x-[AV]-[IMV] Occurences: 8(8) END3_BACSU : 118- 126: vklpg VGRkT-AnVV vsvaf END3_ECOLI : 117- 125: ealpg VGRkT-AnVV lntaf END3_HAEIN : 117- 125: ealag VGRkT-AnVV lntaf GTMR_METTF : 123- 132: ldlpg VGKyTcAaVM clafg MUTY_ECOLI : 117- 125: aalpg VGRsT-AgAI lslsl MUTY_HAEIN : 122- 130: walsg VGRsT-AgAI lssvl MUTY_SALTY : 117- 125: aalpg VGRsT-AgAI lslal UVEN_MICLU : 132- 140: valpg VGRkT-AfVV lgnaf I 9: 25.4104 8( 8) L-x(3)-G-[KR]-x-T-x(0,1)-A-x-[AV]-[IMV] Occurences: 8(8) END3_BACSU : 115- 126: delvk LpgvGRkT-AnVV vsvaf END3_ECOLI : 114- 125: aalea LpgvGRkT-AnVV lntaf END3_HAEIN : 114- 125: ealea LagvGRkT-AnVV lntaf GTMR_METTF : 120- 132: kaild LpgvGKyTcAaVM clafg MUTY_ECOLI : 114- 125: eevaa LpgvGRsT-AgAI lslsl MUTY_HAEIN : 119- 130: eqvwa LsgvGRsT-AgAI lssvl MUTY_SALTY : 114- 125: aeiaa LpgvGRsT-AgAI lslal UVEN_MICLU : 129- 140: edlva LpgvGRkT-AfVV lgnaf J 10: 25.1530 8( 8) L-[APS]-G-V-x(2,3)-T-x(0,2)-A-x-[LV] Occurences: 8(8) END3_BACSU : 115- 125: delvk LPGVgrkT--AnV vvsva END3_ECOLI : 114- 124: aalea LPGVgrkT--AnV vlnta END3_HAEIN : 114- 124: ealea LAGVgrkT--AnV vlnta GTMR_METTF : 120- 131: kaild LPGVgkyTc-AaV mclaf MUTY_ECOLI : 114- 126: eevaa LPGVgrsTagAiL slslg MUTY_HAEIN : 119- 131: eqvwa LSGVgrsTagAiL ssvln MUTY_SALTY : 114- 126: aeiaa LPGVgrsTagAiL slalg UVEN_MICLU : 129- 139: edlva LPGVgrkT--AfV vlgna K 11: 25.0577 8( 8) D-[AGT]-x-[FIV]-x-R-[LV]-x(2)-R-x(3)-[ACGTV]-x(3)-[DNPS] Occurences: 8(8) END3_BACSU : 139- 156: paiav DThVeRVskRlgiCrwkD svlev END3_ECOLI : 138- 155: ptiav DThIfRVcnRtqfApgkN veqve END3_HAEIN : 138- 155: ptiav DThIfRVcnRtnfAagkD vvkve GTMR_METTF : 144- 161: kaamv DAnFvRVinRyfgGsyeN lnynh MUTY_ECOLI : 138- 155: hfpil DGnVkRVlaRcyaVsgwP gkkev MUTY_HAEIN : 143- 160: pypil DGnVkRVlaRyfaVegwS gekkv MUTY_SALTY : 138- 155: hypil DGnVkRVlaRcyaVsgwP gkkev UVEN_MICLU : 153- 170: pgitv DThFgRLarRlgfTdetD pgkgr L 12: 23.8809 8( 8) P-x-C-[ADEGST]-x-C-[GIP]-[ILM]-x(3)-C Occurences: 8(8) END3_BACSU : 194- 205: ckaqs PrCAeCPLlslC regqk END3_ECOLI : 192- 203: ciark PrCGsCIIedlC eykek END3_HAEIN : 192- 203: ciark PrCGsCIIedlC eykek GTMR_METTF : 202- 213: caprk PkCEkCGMsklC syyek MUTY_ECOLI : 197- 208: ctrsk PkCSlCPLqngC iaaan MUTY_HAEIN : 202- 213: cmrtk PkCDlCPLnidC laykn MUTY_SALTY : 197- 208: ctrsk PkCTlCPLqngC iaaah UVEN_MICLU : 208- 219: charr PaCGrCPIarwC psyaa M 13: 23.7241 8( 8) K-[AET]-[AL]-x(2)-V-x(5)-G-x(1,3)-P-x(4)-[ADEQ] Occurences: 8(8) END3_BACSU : 121- 139: pgvgr KTAnvVvsvafGv--PaiavD thver END3_ECOLI : 120- 138: pgvgr KTAnvVlntafGw--PtiavD thifr END3_HAEIN : 120- 138: agvgr KTAnvVlntafGh--PtiavD thifr GTMR_METTF : 96- 116: raeql KELarVvindyGgrvPrnrkA ildlp MUTY_ECOLI : 91- 110: arnlh KAAqqVatlhgGkf-PetfeE vaalp MUTY_HAEIN : 96- 115: arnlh KAAqkVrdefnGnf-PtnfeQ vwals MUTY_SALTY : 91- 110: arnlh KAAqqVatlhgGef-PqtfaE iaalp UVEN_MICLU : 135- 153: pgvgr KTAfvVlgnafGq--PgitvD thfgr N 14: 20.4830 8( 8) L-[APS]-G-x(2,4)-T-x(0,2)-A-x-[LV] Occurences: 8(8) END3_BACSU : 115- 125: delvk LPGvgrkT--AnV vvsva END3_ECOLI : 114- 124: aalea LPGvgrkT--AnV vlnta END3_HAEIN : 114- 124: ealea LAGvgrkT--AnV vlnta GTMR_METTF : 120- 131: kaild LPGvgkyTc-AaV mclaf MUTY_ECOLI : 114- 126: eevaa LPGvgrsTagAiL slslg MUTY_HAEIN : 119- 131: eqvwa LSGvgrsTagAiL ssvln MUTY_SALTY : 114- 126: aeiaa LPGvgrsTagAiL slalg UVEN_MICLU : 129- 139: edlva LPGvgrkT--AfV vlgna O 15: 20.3307 8( 8) A-x(2)-V-x-[DGNST]-x(3)-G-x(1,2)-P-x(4)-[ADEQ] Occurences: 8(8) END3_BACSU : 123- 139: vgrkt AnvVvSvafGv-PaiavD thver END3_ECOLI : 122- 138: vgrkt AnvVlNtafGw-PtiavD thifr END3_HAEIN : 122- 138: vgrkt AnvVlNtafGh-PtiavD thifr GTMR_METTF : 99- 116: qlkel ArvViNdygGrvPrnrkA ildlp MUTY_ECOLI : 93- 110: nlhka AqqVaTlhgGkfPetfeE vaalp MUTY_HAEIN : 98- 115: nlhka AqkVrDefnGnfPtnfeQ vwals MUTY_SALTY : 93- 110: nlhka AqqVaTlhgGefPqtfaE iaalp UVEN_MICLU : 137- 153: vgrkt AfvVlGnafGq-PgitvD thfgr P 16: 19.8307 8( 8) K-x(4,5)-V-x-[DGNST]-x(3)-G-x(1,2)-P-x(4)-[ADEQ] Occurences: 8(8) END3_BACSU : 121- 139: pgvgr Ktanv-VvSvafGv-PaiavD thver END3_ECOLI : 120- 138: pgvgr Ktanv-VlNtafGw-PtiavD thifr END3_HAEIN : 120- 138: agvgr Ktanv-VlNtafGh-PtiavD thifr GTMR_METTF : 96- 116: raeql KelarvViNdygGrvPrnrkA ildlp MUTY_ECOLI : 91- 110: arnlh Kaaqq-VaTlhgGkfPetfeE vaalp MUTY_HAEIN : 96- 115: arnlh Kaaqk-VrDefnGnfPtnfeQ vwals MUTY_SALTY : 91- 110: arnlh Kaaqq-VaTlhgGefPqtfaE iaalp UVEN_MICLU : 135- 153: pgvgr Ktafv-VlGnafGq-PgitvD thfgr Q 17: 19.7108 8( 8) C-[ADEGST]-x-C-[GIP]-[ILM]-x(3)-C Occurences: 8(8) END3_BACSU : 196- 205: aqspr CAeCPLlslC regqk END3_ECOLI : 194- 203: arkpr CGsCIIedlC eykek END3_HAEIN : 194- 203: arkpr CGsCIIedlC eykek GTMR_METTF : 204- 213: prkpk CEkCGMsklC syyek MUTY_ECOLI : 199- 208: rskpk CSlCPLqngC iaaan MUTY_HAEIN : 204- 213: rtkpk CDlCPLnidC laykn MUTY_SALTY : 199- 208: rskpk CTlCPLqngC iaaah UVEN_MICLU : 210- 219: arrpa CGrCPIarwC psyaa R 18: 18.9051 8( 8) L-x(2,4)-R-x-T-A-x(2)-[IV] Occurences: 8(8) END3_BACSU : 115- 126: delvk LpgvgRkTAnvV vsvaf END3_ECOLI : 114- 125: aalea LpgvgRkTAnvV lntaf END3_HAEIN : 114- 125: ealea LagvgRkTAnvV lntaf GTMR_METTF : 44- 53: litei Llr--RtTAghV kkiyd MUTY_ECOLI : 114- 125: eevaa LpgvgRsTAgaI lslsl MUTY_HAEIN : 119- 130: eqvwa LsgvgRsTAgaI lssvl MUTY_SALTY : 114- 125: aeiaa LpgvgRsTAgaI lslal UVEN_MICLU : 129- 140: edlva LpgvgRkTAfvV lgnaf S 19: 15.7350 8( 8) R-x-T-A-x(2)-[IV] Occurences: 8(8) END3_BACSU : 120- 126: lpgvg RkTAnvV vsvaf END3_ECOLI : 119- 125: lpgvg RkTAnvV lntaf END3_HAEIN : 119- 125: lagvg RkTAnvV lntaf GTMR_METTF : 47- 53: eillr RtTAghV kkiyd MUTY_ECOLI : 119- 125: lpgvg RsTAgaI lslsl MUTY_HAEIN : 124- 130: lsgvg RsTAgaI lssvl MUTY_SALTY : 119- 125: lpgvg RsTAgaI lslal UVEN_MICLU : 134- 140: lpgvg RkTAfvV lgnaf T 20: 14.6802 8( 8) G-x-V-x(1,3)-R-x(1,3)-L Occurences: 8(8) END3_BACSU : 103- 112: iedyg GeVprdRde-L vklpg END3_ECOLI : 102- 111: leqhn GeVpedRaa-L ealpg END3_HAEIN : 102- 111: iekhn GeVpenRea-L ealag GTMR_METTF : 108- 118: indyg GrVprnRkaiL dlpgv MUTY_ECOLI : 139- 145: fpild GnVk--Rv--L arcya MUTY_HAEIN : 144- 150: ypild GnVk--Rv--L aryfa MUTY_SALTY : 139- 145: ypild GnVk--Rv--L arcya UVEN_MICLU : 117- 126: vgrhd GeVpa-RledL valpg Number of patterns evaluated by Pratt:2460 Total running time: 1 seconds